During haematopoiesis, haematopoietic stem cells differentiate into restricted potential progenitors before differentiating into the many lineages required for oxygen transport, wound healing and immune response. We have created Haemopedia (1), a database of gene-expression profiles from a broad spectrum of haematopoietic cells, which includes RNA-seq gene-expression data from both mice and humans. The Haemopedia RNA-seq dataset covers a wide range of lineages and progenitors, with 57 mouse blood cell types (flow sorted populations from healthy mice) and 12 human blood cell types. This dataset has been made accessible for exploration and analysis, to researchers and clinicians with limited bioinformatics experience, on our online portal Haemosphere: www.haemosphere.org. Haemosphere also includes 11 other publicly available high-quality data sets relevant to haematopoiesis. Users can compare gene expression across datasets and species using shared lineage designations, perform differential expression or view expression gene vs gene, with all plots available for download by the user.