The screening of large populations and the associated challenges in automation, chemistry and cost are major scientific but also business drivers in plant breeding. Genotyping methods such as KASP® or based on BHQ® probes are widespread, but require a previous, time-consuming, labour- and cost-intensive lysis of the plant cells and subsequent extraction of the DNA. QuickExtract™ DNA Extraction Solution provides a fast, simple, and inexpensive method for preparing genomic DNA for endpoint PCR amplification without the use of toxic chemicals. DNA extraction requires only heat treatment to lyse the sample material, release the DNA, and degrade compounds inhibitory to amplification. Following heat treatment, the sample DNA is ready for PCR in less than 15 min. The procedure is convenient and can be easily scaled to process hundreds of samples in multiwell plates, using robotic automation systems.
The grinding of plant samples such as leaves or seeds is an important step in the extraction of DNA and has a direct impact on extraction specifications like DNA quantity, quality and molecular weight.
Successful genotyping also often depends on a variety of different factors, such as the plant species to be investigated, the sample material, the lysis conditions or dilutions and incubations. This study investigates the influence of grinding on the quality of genotyping results of 6 different plant species and plant samples in total. Also genotyping methods such as KASP or BHQ probes were tested.